Silica Based Cartridges

Clinisphere® C18

  • Clinisphere C18 is the most broadly used SPE cartridge. It can be used to adsorb non-polar, slightly polar and mid-polar compounds.
  • Polar materials such as salt cannot be retained on the sorbent, which makes ProElut™ C18 an excellent choice for desalting samples. In addition, non-polar and slightly polar disruptors in matrix such as fats, PAHs.
  • Phthalates can be retained by the sorbent, leaving ionic analytes eluted in the collector for reconstitution.

Clinisphere® C8

  • Clinisphere C8 is very similar to the C18 phase, but has a shorter chain. This makes the phase less non-polar than C18, leaving non-polar compounds less retained by the sorbents.
  • In this case, those compounds retained too strongly on the C18 can be effectively eluted if you choose the C8 phase.
  • In addition, the C8 phase for polar interaction is somewhat higher than C18 because there is less coverage of the silica surface. However, this polar interaction is not the main characteristic of C8 phase.

Clinisphere® PH

  • Clinisphere PH has a similar polarity as that of C8 phase. However, it shows different selectivity to other non-polar phases due to the presence of conjugated double bonds.
  • In addition, the planar shape of benzene and its electron distribution make it much more retentive to aromatic compounds.

Clinisphere® CN

  • Clinisphere CN is a mid-polar phase SPE column.
  • We recommend using it to extract samples for which analytes are irreversibly retained on C8 and C18 phases.

Clinisphere® Silica

  • Clinisphere Silica is the most polar SPE sorbent.
  • It is very effective for separating compounds with similar structures and extracting polar compounds in non-polar solvents.
  • The silica surface silanols have slight anion exchange properties that can be used to remove organic acids and phenols in extracts.

Clinisphere® NH2

  • Clinisphere NH2 has both polar and weak anion exchange interactions.
  • It can effectively absorb compounds with a polar functional group (-OH, -NH2,-SH, etc.) by hydrogen bonding from non-polar solvents such as hexane.
  • In addition, it has weaker anion exchange property than SAX (a quaternary amine sorbent that is always charged) and is therefore an excellent choice for retention of very strong anions that are always irreversibly adsorbed on a SAX sorbent, such as sulfonic acid.

Clinisphere® SCX

  • Clinisphere SCX sorbent has benzenesulfonic acid as a bonded functional group with a very low pKa.
  • The presence of the benzene ring in the functional group increases its potential for non-polar interaction.
  • The two properties are quite useful in the absorption of cationic organic compounds from aqueous systems where non-polar compounds are seen.

Clinisphere® SAX

  • Clinisphere SAX sorbent has trimethylaminopropyl as a bonded functional group with a very high pKa.
  • The presence of the benzene ring in the functional group increases its potential for non-polar interaction.
  • The two properties are quite useful in the absorption of anionic organic compounds from aqueous systems where non-polar compounds are seen.
Clinisphere
C18		 Clinisphere
C8		 Clinisphere
PH		 Clinisphere
CN		 Clinisphere
Silica		 Clinisphere
NH2		 Clinisphere
SCX		 Clinisphere
SAX
Base material Spherical silica, Particle Size: 50 μm, Pore Size: 60 Å, Specific Surface Area: 500 m2/g Spherical silica, Particle Size: 50 μm, Pore Size: 60 Å, Specific Surface Area: 500 m2/g Spherical silica, Particle Size: 50 μm, Pore Size: 60 Å, Specific Surface Area: 500 m2/g Spherical silica, Particle Size: 50 μm, Pore Size: 60 Å, Specific Surface Area: 500 m2/g Spherical silica, Particle Size: 50 μm, Pore Size: 60 Å, Specific Surface Area: 500 m2/g Spherical silica, Particle Size: 50 μm, Pore Size: 60 Å, Specific Surface Area: 500 m2/g Spherical silica, Particle Size: 50 μm, Pore Size: 60 Å, Specific Surface Area: 500 m2/g Spherical silica, Particle Size: 50 μm, Pore Size: 60 Å, Specific Surface Area: 500 m2/g
Functional group
End capping Yes Yes Yes Yes No No No No
Carbon loading 19% 11% 10% 8% - 5.5% 10.9% 8%
Retention mechanism Reversed phase Reversed phase Reversed phase Reversed phase or normal phase Normal phase or weak anion exchange Normal phase or anion exchange Strong cation exchange Strong anion exchange
Application Extraction of non-polar to moderately polar compound such as antibiotics, barbiturates benzodiazepines, caffeine, drugs, dyes, essential oils, fat-soluble vitamins, fungicides For reversed phase extraction of non-polar to moderately polar compounds, such as antibiotics, barbiturates, benzodiazepines, caffeine, drugs, dyes. Volatiles in water: PAHs, PAEs, PCBs, pesticides, herbicides, phenols Biological fluids: blood, urine Pesticides in water, metabolites Extract polar compounds from non-polar matrix Remove polar hydrocarbons, organic acids, and phenols in extracts. Separate compounds with very similar structures (isomers) Extract polar compounds from non-polar matrix Remove polar hydrocarbons, organic acids, and phenols in extracts Separate compounds with very similar structures (isomers) Basic compounds in aqueous solution Carboxylic acids in aqueous solution

More material with respect to application will available on request.

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